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张琦, 臧巧路, 林新春. 赛山梅组培快繁技术初探[J]. 浙江林业科技, 2017, 37(1): 51-54. DOI: 10.3969/j.issn.1001-3776.2017.01.009
引用本文: 张琦, 臧巧路, 林新春. 赛山梅组培快繁技术初探[J]. 浙江林业科技, 2017, 37(1): 51-54. DOI: 10.3969/j.issn.1001-3776.2017.01.009
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ZHANG Qi, ZANG Qiao-lu, LIN Xin-chun. Experiment on Microprapagation of Styrax confuses[J]. Journal of Zhejiang Forestry Science and Technology, 2017, 37(1): 51-54. DOI: 10.3969/j.issn.1001-3776.2017.01.009
Citation: ZHANG Qi, ZANG Qiao-lu, LIN Xin-chun. Experiment on Microprapagation of Styrax confuses[J]. Journal of Zhejiang Forestry Science and Technology, 2017, 37(1): 51-54. DOI: 10.3969/j.issn.1001-3776.2017.01.009
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赛山梅组培快繁技术初探

Experiment on Microprapagation of Styrax confuses

    摘要
  • 摘要: 以赛山梅Styrax confusus 的带芽茎段为外植体，MS 为基本培养基，对其进行微繁实验。结果表明，赛山梅的最佳增殖培养基为MS+1 mg·L-1BA，其最高增殖系数为4.00，且芽丛生长旺盛；最佳生根培养基为1/2MS +1mg·L-1IBA，生根率为40%，根系较长且健壮；试管苗移栽至泥炭、蛭石、珍珠岩配制比例为1:1:1 的混合基质中，成活率为58%。

     

    Abstract: Experiments were conducted on tissue culture of Styrax confuses by stem with bud as explants. The results showed that MS supplementedwith 1 mg/L of BA had the best effect for bud multiplication, with the highest proliferation coefficient of 4.00, and the bud cluster grew well. The bestmedium for rooting was 1/2 MS supplemented with 1 mg/L of IBA, with rooting rate up to 40%, and the roots were long and strong. Plantlets weretransplanted in medium of peat, vermiculite and perlite with a ratio at 1:1:1. Survival rate was 58% after 4 weeks.

     

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