Abstract: Investigations were conducted from June 2014 to May 2016 on gymnosperm resources in the southwest Hubei province, by route surveyand sample plot one. Endangered degree and genetic loss coefficient of the resources were analyzed by endangered index and urgency value, and theirutilization was classified. The results show that there were 89 species of 9 families and 33 genera of gymnosperms in southwest Hubei (including 10varieties and 12 cultivars). Among them, 36 species are endemic, accounting 40.4% of the total species of gymnosperms in the southwest Hubei.There were 12 species under national key protection, 10 species of national rare and endangered, 13 species of provincial rare and endemic.Gymnosperm resources in the southwest Hubei with endangered index and urgency value of less than 0.5 occupied 33.3% and 38.9% of the totalregional natural distribution species, indicating gymnosperm resources in the southwest Hubei was threatened. Gymnosperm resources in the regionwere classified into 14 categories, including wood, ornamental and medicinal. According to endangered degree and the urgency value of gymnospermresources in the southwest Hubei province, recommendations were put forwarded.