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李海波, 刘勇, 赵佳, 王燕飞, 徐梁, 杨明. 12 个油茶良种的SCAR 分子标记鉴别[J]. 浙江林业科技, 2017, 37(2): 17-23. DOI: 10.3969/j.issn.1001-3776.2017.02.003
引用本文: 李海波, 刘勇, 赵佳, 王燕飞, 徐梁, 杨明. 12 个油茶良种的SCAR 分子标记鉴别[J]. 浙江林业科技, 2017, 37(2): 17-23. DOI: 10.3969/j.issn.1001-3776.2017.02.003
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LI Hai-bo, LIU Yong, ZHAO Jia, WANG Yan-fei, XU Liang, YANG Ming. Identification of 12 Superior Cultivars of Camellia oleifera by Sequence CharacterizedAmplified Region Markers[J]. Journal of Zhejiang Forestry Science and Technology, 2017, 37(2): 17-23. DOI: 10.3969/j.issn.1001-3776.2017.02.003
Citation: LI Hai-bo, LIU Yong, ZHAO Jia, WANG Yan-fei, XU Liang, YANG Ming. Identification of 12 Superior Cultivars of Camellia oleifera by Sequence CharacterizedAmplified Region Markers[J]. Journal of Zhejiang Forestry Science and Technology, 2017, 37(2): 17-23. DOI: 10.3969/j.issn.1001-3776.2017.02.003
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12 个油茶良种的SCAR 分子标记鉴别

Identification of 12 Superior Cultivars of Camellia oleifera by Sequence CharacterizedAmplified Region Markers

    摘要
  • 摘要: 以12 个长林系列油茶Camellia oleifera 良种为试材，基于特异性SCAR 分子标记的开发来鉴别油茶品种。结果表明：基于4 条SAPD 引物、24 对SRAP 引物和15 个ISSR 引物分别对12 个油茶品种进行扩增，得到了21条多态性片段，包括7 条nSAPD，10 条SRAP 和4 条ISSR 片段。2 条nSAPD，7 条SRAP 和2 条ISSR 多态性片段被成功转化为了11 个稳定可靠的SCAR 标记。长林油茶23 号、长林26 号、长林53 号、长林56 号和长林166 号有1 个SCAR 标记，长林3 号和长林55 号各有2 个SCAR 标记，长林21 号有4 个SCAR 标记，长林18号有6 个SCAR 标记，长林4 号、长林27 号和长林40 号没有SCAR 标记。这些SCAR 标记可作为品种特异性的DNA 指纹，辅助用于12 个长林系列油茶良种的鉴别。

     

    Abstract: Experiments were conducted on sequence characterized amplified region markers (SCAR) from amplified polymorphic DNA (SAPD),sequence-related amplified polymorphism and inter simple sequence repeat (ISSR) molecular techniques for identification of 12 cultivars (Changlinseries)of Camellia oleifera. The result demonstrated that twenty-one polymorphic bands (7 from nSAPD, 10 from SRAP and 4 from ISSR) wereobtained from the tested cultivars by using 43 primers (4 SAPD, 24 SRAP and 15 ISSR). Eleven (2 from nSAPD, 7 from SRAP and 2 from ISSR) of21 polymorphic bands were converted into 11 stable and reproducible SCAR markers by designing specific SCAR primers from the 11 sequencedpolymorphic bands and by verifying PCR amplification with SCAR primers. Changlin 23, 26, 53, 56 and 166 had only one SCAR marker, Changlin 3and 55 had two ones, Changlin 21 had four ones, Changlin 18 had six ones, and Changlin 4, 27 and 40 have no SCAR markers. These SCAR markerscan be used as cultivar-specific DNA fingerprints for better identification of cultivars.

     

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