Abstract: Permanent sample plots were established in 2006 at Phyllostachys heterocycla cv. pubescens stands in 11 counties from the main producingareas of Zhejiang province. Different fertilization treatments were conducted on bamboo stands like application of urea, compound fertilizer, organicfertilizer and no fertilization (ck) to determine pH during 2006-2015. The results showed that the mean annual soil acidification rate of the controlwas 0.011 pH, and that of the plots treated by urea reached 0.082 pH, about 7.5 time of the CK. The annual average soil acidification rate of standtreated with compound fertilizer was 0.053 pH, which was 4.8 times of the CK, and that of stands treated by organic fertilizer was 0.019 pH, 1.7 timeof the CK. Therefore, suggestions were offered such as reducing application of chemical fertilizers instead of ecological fertilization and managementfor healthy and sustainable development of bamboo forest.