超声波协同复合酶法提取桑黄多糖工艺优化

Ultrasonic-assisted Enzymatic Method Extraction of Polysaccharide from Inonotus sanghuang

摘要: 在单因素试验的基础上，通过Box-Benhnken组合设计和响应曲面分析法优化桑黄Inonotus sanghuang子实体中多糖的超声波协同复合酶法提取工艺条件。在前期预实验的基础上，采用纤维素酶、果胶酶和蛋白酶按2:1:1组成复合酶对桑黄子实体进行酶解处理，添加量为2%，温度50℃，pH 6.5，在水溶液中酶解1 h。然后对工艺中的超声波功率、超声时间、液料比进行优化。响应面分析结果表明，桑黄子实体中多糖的最佳提取条件为：超声功率360.6 W，液料比32.5:1，超声时间32.7 min，多糖得率可得3.31%，与理论值3.46%基本吻合。

Abstract: Extraction technology of polysaccharide from Inonotus sanghuang was optimized by ultrasonic-assisted enzymatic method and response surface analysis. Based on the results of single factor experiment, enzymolysis of I. sanghuang sporcarp was carried out with 2% of complex phosphoesterasum (ratio of cellulose, pectinase, protease of 2:1:1) at 50℃ under pH of 6.5 for 1 hour. Then optimization of extraction technology was implemented on ultrasonic power, ultrasound time and ratio of liquid and material. The response surface analysis demonstrated that polusaccharide yield topped 3.31% under condition as follows: ultrasonic power 360.6W, liquid/material ratio 32.5:1, ultrasound treatment time 32.7minutes, similar to theoretical value of 3.46%.