Abstract: Experiments were conducted from May 2014 to December 2015 on different substrates and different fertilizer to cultivate 2-year Osmanthus fragrans, 3-year Ginkgo biloba and Michelia chapensis. The results showed that substrate of sub-top soil: peat: pearlite = 2:2:1 could significantly promote the growth of tested species and was recommended for sapling cultivation with lower cost and better adaptivity Different fertilization experiments showed that saplings of O. fragrans and G. biloba grew better with both 1.5 kg·m-3 of slow-release fertilizer (SRF) and 3.5 kg·m-3 of compound fertilizer (CF), with advantages of less quantity for fertilization but little environment pollution. The growth of M. chapensis was better with each quantity of SRF than that with CF, and it had the best growth with 3.5 kg·m-3 of SRF, showing positive relation with quantity of SRF.