超声波辅助提取桑黄黄酮工艺优化

Optimizing Technology for Ultrasonic Extraction of Total Flavonoidsfrom Inonotus sanghuang

摘要: 选择乙醇体积分数、超声时间、超声温度3 个因素，通过Box-Benhnken 组合设计和响应曲面分析法优化桑黄纤孔菌Inonotus sanghuang 子实体中总黄酮的超声波辅助提取工艺条件。结果表明，桑黄子实体中总黄酮的最佳提取条件为：乙醇体积分数70.9%，超声时间47.8 min，超声温度55.2℃，总黄酮的得率理论值达2.63%。验证试验表明，总黄酮得率平均为2.51%，与理论值基本吻合。

Abstract: Extraction technology of flavonoids from Inonotus sanghuang was optimized by ultrasonic-assisted method. Based on the results ofone-factor-at-a-time-technique method, a three-level Box-Behnken factorial design was implemented combining with response surfacemethodologyto optimize the extraction conditions of total flavonoids. The result demonstrated that theoretical yield of flvonoids could reach 2.63%underultrasonic temperature of 55.2℃, ethanol concentration of 70.9% and ultrasound treatment time of 47.8minutes. Verification test resulted theaverage yield of 2.51%, similar to theoretical value.