Abstract: In November 2015, collections of lateral branches of Dendrocalamopsis oldhami were carried out in Dongshan county, Zhangzhou of Fujian province for experiment of rooting of cuttings. Cuttings were treated with 600 mg/L of ABT1 for 5 min, and planted in medium of yellow soil: peat soil =1: 1. Determinations and observation were implemented on rooting properties and changes of soluble sugar content, soluble protein content, and some antioxidant enzymes activity during rooting time. The results showed that treated cuttings had rooting rate of 57.25%. During the process of rooting, the content of soluble sugar decreased first, then increased, and decreased at last. The changes of soluble protein content showed the opposite trend. The activity of peroxidase（POD）, indole acetic acid oxidase（IAAO）, polyphenol oxidase（PPO）and superoxide dismutase（SOD）increased during 0－14th day after planting, and the activity of POD and PPO decreased, that of IAAO increased while that of SOD stable during the 14th －21st day. Activities of IAAO and PPO declined, of POD increased first and then decreased and of SOD slightly increased during the 21st－35th day.