Abstract: In July 2017, two sample plots of 60 m × 50 m were established at secondary forest in Changpoling Forest Farm, Guizhou province for analysis of spatial structure by mingling degree, neighborhood pattern and comparison. The result demonstrated that arbor layer was mainly composed by 13 species, such as Celtis sinensis, Bothrocaryum controversum, Ligustrum lucidum, etc. Analysis on mingling degree indicated that the two sample plots were intensively mixed with few same species neighboring. Mean neighborhood pattern was 0.475-0.517, indicating random distribution. The neighborhood comparison an different level of the stand was uniform, meaning middle state.