NaCl 胁迫对海滨木槿幼苗AsA-GSH 循环的影响

孙 慧; 吴 华; 张 振; 韩 榆; 俞慈英; 杜希华

doi:10.3969/j.issn.1001-3776.2018.02.009

NaCl 胁迫对海滨木槿幼苗AsA-GSH 循环的影响

Effects of NaCl Stress on AsA-GSH Cycle in Seedlings of Hibiscus hamabo

摘要

摘要: 研究了海滨木槿Hibiscus hamabo 子叶期幼苗和早期幼苗抗坏血酸-谷胱甘肽循环对NaCl 胁迫的响应规律，结果显示，经150 和300 mmol·L-1NaCl 处理的海滨木槿SOD（超氧化物歧化酶），APX（抗坏血酸过氧化物酶）和GR（谷胱甘肽还原酶）活性较对照显著提高。对子叶期幼苗，各指标呈现先升高后降低趋势；AsA（抗氧化剂抗坏血酸），GSH（谷胱甘肽）含量随盐浓度升高呈下降趋势，O2 ·-的产生速率、H2O2 含量与盐浓度呈正相关。而早期幼苗中SOD，APX 和GR 活性以及AsA，GSH 含量均随NaCl 浓度升高而升高，O2 ·-的产生速率、H2O2 含量也随NaCl 浓度的增加不断上升。实验结果表明，随着海滨木槿幼苗的生长发育，海滨木槿通过提高AsA-GSH 循环中抗氧化酶活性及抗氧化物质含量来清除因盐胁迫产生的活性氧，增强对盐胁迫产生的活性氧的清除。

Abstract: In November 2013, seeds of Hibiscus hamabo were harvested in Zhoushan, Zhejiang province, and in next June and July, sand cultured for experiment on response of ascorbate-glutathione (AsA-GSH) cycle of early stage seedlings under different concentrations of NaCl stress. The results showed that the activity of superoxide dismutase (SOD), ascorbic acid peroxidase (APX) and glutathione reductase (GR) of seedlings treated by 150 and 300 mmol·L-1NaCl was significantly higher than that of the control (no treatment), and of seedlings with cotyledons increased at first and then decreased. The content of AsA and GSH had negative relation with the concentration of NaCl, while O2 ·- production rate and H2O2 content had positive one. However, in the early stage seedlings, as high salinity buoyed up the O2 ·- production rate and H2O2 level, the activity of SOD, APX and GR and the content of AsA and GSH were increased with the concentration of NaCl.

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