Abstract:
New shoots were collected from 14-year Cinnamomum camphora‘Yongjin’ in Ningbo, Zhejiang province in March of 2015 for experiment on propagation of clump buds. In order to improve the proliferation efficiency and growth condition of clump buds in C.caphora‘Yongjin’, new shoots of axillary buds that have not yet germinated was as experimental materials of the‘Yongjin’in this paper, systematic study was carried out about different medium, hormones, sucrose concentrations, pH values, subculture patterns and succession subculture cycles by means of plant tissue culture techniques was tested on growth of clump buds. The result showed that the optimal scheme propagation of clump buds was as follows: MS+50 mg·L-1 of NaH2PO4+2 mg·L-1 of 6-BA+0.02 mg·L-1of IBA, about multiplication optimization of clump buds in‘Yongjin’, and sucrose concentration was between 20-30 g·L-1, pH was 5.8-6.2, subculture pattern was ①-③-①(①: MS+50 mg·L-1 of NaH2PO4+3 mg·L-1 of 6-BA+0.02mg·L-1 of 6- IBA,③: MS+50 mg·L-1 of NaH2PO4+1 mg·L-1 of 6-BA+0.02 mg·L-1 of IBA, subculture cycle was of 25-30 days. The results of this study can effectively improve growing weakness, yellow, brown of the buds in ‘Yongjin’ subculture, and increase the proliferation efficiency.