Abstract: Fresh rhizomes of Polygonatum cyrtonema were harvested and cut into segments in November of 2014 under Castanea henryi stand. In March of 2015, cuttings were planted with different methods for comparison on their growth. The results showed that there was no difference of phonological period of seedlings among different methods, but evident among different sample plots. Seedling height and leaf number of seedlings by common planting was significantly higher than that by the other planting methods. There was no significant difference of stem diameter, weight of rhizomes and bud number of seedlings by different planting methods, but weight of rhizome of seedlings by different planting methods was 9.93% and 9.28% higher than that of common planting. The experiment demonstrated that seedling cultivation of P. cyrtonema could be implemented by different methods.