Abstract: Experiment of selection of formula for cultivation substrate for high yield of Dictyophora echinovolvata was carried out in 2015 under Castanea henryi stand in Qingyuan, Zhejiang province. Next year, further experiments were implemented on different cultivation time, substrate quantity, fertilization of C. henryi stand, furrowing and non-furrowing on yield of D. echinovolvata. The result showed different cultivation substrate formula, cultivation time, substrate quantity and fertilization had significant effect on yield of D. echinovolvata, but furrowing and non-furrowing had no evident influence. The experiment showed that fermented substrate, cultivation in February with unit area substrate of 37.5 kg·m-2 had better effect. C. henryi stand with low fertility was needed to be fertilized, and with low hardness could cultivate with non-furrowing.