Abstract: Collections of 95 germplasm resources of Cerasus spp. were carried out in May and June of 2016 from 14 counties of Zhejiang province. Experiments were implemented for their genetic diversities using 17 ISSR (inter simple sequence repeat) markers from 100 ones. The results showed that 110 bands were amplified by 17 primers, in which 101 bands were polymorphic with average percentage of ISSR polymorphism of 91.78 %. The genetic similarity coefficients of tested resources ranged from 0.38 to 0.90, with an average of 0.61. The genetic distances ranged from 0.10 to 1.38, with an average of 0.5. UPGMA (unweighted pair-group method with arithmetic means) analysis demonstrated that 95 germplasm resources could be divided into four categories when genetic similarity coefficient was 0.5775. The results indicated that there were rich genetic diversities in Cerasus spp. from Zhejiang.