松材线虫果胶酶基因Bxpel1的克隆及其生物信息学分析

邱秀文; 周桂香; 王慧娟; 杨丽丽

doi:10.3969/j.issn.1001-3776.2018.04.002

松材线虫果胶酶基因Bxpel1的克隆及其生物信息学分析

Cloning and Bioinformatics Analysis of Bxpel1 Gene from Bursaphelenchus xylophilus

摘要

摘要: 以松材线虫Bursaphelenchus xylophilus 转录本为模板，通过RT-PCR 技术克隆果胶酶Bxpel1 基因。结果表明，松材线虫果胶酶Bxpel1 基因序列全长为795 bp，GC 含量为50.44%，编码252 个氨基酸。通过Blast 比对克隆的Bxpel1 基因与已知序列（Gen-Bank ID：AB232908.1）的同源性达到98%。系统进化分析表明，Bxpel1 基因编码产物的氨基酸序列与拟松材线虫Bursaphelenchus mucronatus，燕麦真滑刃线虫Aphelenchus avenae 的Bxpel1 蛋白序列具有高度同源性。Bxpel1 基因编码的产物的0 ~ 25 序列有可能是跨膜结构域，而其他序列均位于膜外，其二级结构主要是以无规则卷曲和β-折叠为主，信号肽的剪切位点位于第17 至第18 位氨基酸之间，主要在细胞外发挥生物学作用。Bxpel1 基因的克隆及其生物信息学分析对进一步研究Bxpel1 基因在松材线虫致病过程中的功能具有重要意义。

Abstract: Bxpel1 gene sequence from Bursaphelenchus xylophilus cDNA as a template was amplified and cloned by RT-PCR. Bxpel1 gene (795bp) was successfully cloned with GC content of 50.44% and encode of 252 amino acids. The cloned sequence had 98% similarity to the pel1 gene previously published in the GenBank (ID: AB232908.1). Phylogenetic analysis showed that Bxpel1 had high homologies with that from B. mucronatus and Aphelenchus avenae. Bxpel1 gene encode from 0 to 25 sequences may be transmembrane structure, while the other sequences outside membrane. The secondary and tertiary structures were abundant in random coils and beta helix. The signal peptide cleavage sites locate between No. 17 to No. 18 amino acid and performs biological effects at extracellular.

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