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秦玉川, 王丽玲, 王衍彬, 贺亮, 刘本同, 钱华, 童晓青. 大苞鞘石斛中多糖DWW-1的提取纯化及其结构与性质分析[J]. 浙江林业科技, 2018, 38(4): 15-20. DOI: 10.3969/j.issn.1001-3776.2018.04.003
引用本文: 秦玉川, 王丽玲, 王衍彬, 贺亮, 刘本同, 钱华, 童晓青. 大苞鞘石斛中多糖DWW-1的提取纯化及其结构与性质分析[J]. 浙江林业科技, 2018, 38(4): 15-20. DOI: 10.3969/j.issn.1001-3776.2018.04.003
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QIN Yu-chuan, WANG Li-ling, WANG Yan-bin, HE Liang, LIU Ben-tong, QIAN Hua, TONG Xiao-qing. Purification and Analysis of DWW-1 from Dendrobium wardianum[J]. Journal of Zhejiang Forestry Science and Technology, 2018, 38(4): 15-20. DOI: 10.3969/j.issn.1001-3776.2018.04.003
Citation: QIN Yu-chuan, WANG Li-ling, WANG Yan-bin, HE Liang, LIU Ben-tong, QIAN Hua, TONG Xiao-qing. Purification and Analysis of DWW-1 from Dendrobium wardianum[J]. Journal of Zhejiang Forestry Science and Technology, 2018, 38(4): 15-20. DOI: 10.3969/j.issn.1001-3776.2018.04.003
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大苞鞘石斛中多糖DWW-1的提取纯化及其结构与性质分析

Purification and Analysis of DWW-1 from Dendrobium wardianum

    摘要
  • 摘要: 从大苞鞘石斛Dendrobium wardianum 中分离纯化得到一种均一多糖组分DWW-1，采用化学和光谱学的方 法对其理化性质及初步结构进行研究。首先采用水提醇沉方法制备大苞鞘的粗多糖，经过Sevag 法除蛋白， DEAE-Cellulose 和Sephadex G-75 柱层析分离纯化得到均一多糖DWW-1 纯品。其次利用化学法、高效凝胶过滤 色谱法、液相色谱法，并结合红外光谱，对DWW-1 进行性质及结构分析。结果显示DWW-1 为白色絮状固体， 易溶于水，分子量为6.479×104，糖基组成为葡萄糖、糖醛酸、甘露糖、岩藻糖四种单糖；多糖糖苷键以1→4 连 接键为主，1→3 为次；其次DWW-1 具有较强DPPH 自由基清除活性。初步研究结果表明从大苞鞘石斛中分离到 的是一种具有抗氧化活性多糖，并简称为DWW-1。

     

    Abstract: Crude polysaccharides from Dendrobium wardianum were extracted by water and precipitated by ethanol. Deproteinization with Sevage method, DWW-1 was separated and purified by DEAE-Cellulose and Sephadex G-75 column chromatography. Analysis of properties and structure was performed by using chemical method, HPGPC, HPLC, GC-MS and IR. The results showed that DWW-1 was a white flocculent solid after vacuum freeze-drying, soluble in water, with relative molecular mass of 6.479×104. It was composed of glucose, glucuronic acid, mannose and fucose. DWW-1 showed strong scavenging ability on DPPH free radicals.

     

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