Abstract: Experiments were implemented with different interplantation of different Chinese herbals, different soil preparation intension and different coverage for studying their effect on species diversity in the Castanea henryistands planted in 2003.Investigation on species under C.henryi with different treatments was carried out in June of 2016.Comprehensive evaluation on species diversity was analyzed by Shannon-Wiener diversity index, Margalef richness index, Pielou evenness index and important value index.The results showed that the treatment of interplanting P.cyrtonema with 60% of soil preparation and covered with bamboo saw dust had the highest comprehensive species index.The order of factors influencing species diversity index was interplantation> coverage>soil preparation intension.The experiments showed that the optimal treatmentwas interplanting P.cyrtonemawith soil preparation intensionof 60%, covered with 2cm bamboo saw dust.It had 12 different species of plant under C.henryi.