Abstract: Observations and samplings were carried out during 2012 to 2015 on flower bud of Rubus chingii in Dexing, Jiangxi province.Determinations were implemented by paraffin section, and annual air temperature, insolation duration, precipitation and relation humidity were recorded.The results showed that the flower bud differentiation stages were correlated with annual temperature, sunshine, rainfall and relative humidity duration change.One-year branches of R.chingii were mainly for vegetative growth from spring to early summer.The mixed buds were formed from current branches in early summer.Flower buds were undifferentiated in August and September, calyx primordium was differentiated in early October, petal rimordium in mid-October, stamen primordium in early November and pistil primordium in mid-November.The flower buds growth was fast in the early stage, slower in the middle-late stage until dormancy.After about two months of dormancy, they sprouted in February the next year and blossomed in March.The experiment demonstrated that flower bud differentiation of R.chingii had important relation with relatively low temperature, long insolation duration, lower precipitation and lower relative humidity.