Abstract: Field investigation, specimen identification and reference review was implemented during 2008 and 2016 on Lycophytes in Zhejiang province.The results showed that there were 28 species of 3 families (Huperziaceae, Lycopodiaceae and Selaginella) and 7 genera (Huperzia, Phylegmariurus, Lycopodiastrum, Diphasiastrum, Palhinhaea, Lycopodium and Selaginella), among them, 24 medicinal genera, 85.7% of the total Lycophytes in the province.Lycophytes in Zhejiang belong to the world wide type, the distribution of genera could be divided into three types, but the world wide type occupied the absolute dominance.Presentations were made on organs, functions and application the medicinal Lycophytes. Suggestions were put forwarded on further development of the resources, such as introduction and domestication, deep processing technology.