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李修鹏, 沈登锋, 徐沁怡, 章建红, 何立平, 李东宾, 余敏芬, 沈波. 不同贮藏方法对华顶杜鹃花粉活力的影响[J]. 浙江林业科技, 2019, 39(5): 72-76. DOI: 10.3969/j.issn.1001-3776.2019.05.011
引用本文: 李修鹏, 沈登锋, 徐沁怡, 章建红, 何立平, 李东宾, 余敏芬, 沈波. 不同贮藏方法对华顶杜鹃花粉活力的影响[J]. 浙江林业科技, 2019, 39(5): 72-76. DOI: 10.3969/j.issn.1001-3776.2019.05.011
LI Xiu-peng, SHEN Deng-feng, XU Qin-yi, ZHANG Jian-hong, HE Li-ping, LI Dong-bin, YU Min-feng, SHEN Bo. Effects on Pollen Viabilities of Rhododendron huadingense with Different Storage Methods[J]. Journal of Zhejiang Forestry Science and Technology, 2019, 39(5): 72-76. DOI: 10.3969/j.issn.1001-3776.2019.05.011
Citation: LI Xiu-peng, SHEN Deng-feng, XU Qin-yi, ZHANG Jian-hong, HE Li-ping, LI Dong-bin, YU Min-feng, SHEN Bo. Effects on Pollen Viabilities of Rhododendron huadingense with Different Storage Methods[J]. Journal of Zhejiang Forestry Science and Technology, 2019, 39(5): 72-76. DOI: 10.3969/j.issn.1001-3776.2019.05.011

不同贮藏方法对华顶杜鹃花粉活力的影响

Effects on Pollen Viabilities of Rhododendron huadingense with Different Storage Methods

  • 摘要: 研究了华顶杜鹃Rhododendron huadingense花粉活力水平及不同贮藏方法对其活力的影响。结果表明,新鲜华顶杜鹃花粉的萌发率高达98.1%;室温存放的新鲜花粉在3d内可保持较高的活力,3 d后其活力下降迅速;4℃贮藏7 d后的花粉萌发率仍保持在85.5%的较高水平,贮藏15 d时的萌发率仍高于50%,可作为华顶杜鹃花粉的短期(15 d内)保存方法;-20℃贮藏40 d后的花粉萌发率仍能达到87.4%,基本可满足实际需求;-80℃下贮藏60 d后萌发率依旧维持在95.2%,可用于华顶杜鹃花粉的长期保存。本研究结果说明花粉活力不是导致华顶杜鹃濒危的生物学原因。

     

    Abstract: The pollen viability of Rh.huadingense and the effects ofdifferent storage methodswere studied, the results showed that the germination rate of fresh pollens was as high as 98%, fresh pollens which were stored at room temperature (20℃) had a high activity in 3 days, then the activity of pollens declined rapidly, thegermination rate was about 85.5% when the pollens were stored at 4℃ for a week, after half a month ,the germination rate was up to 50%., It can be used as a short-term (within 15 days) preservation method for Rh. huadingense pollen. When stored at-20℃ for 40 days, the germination rate of pollens was up to 87.4%, while stored at-80℃ for 60 days,the germination ratecould remain at 95.2%, it could be used for the long-term preservation of the pollen.Our results indicate that pollen viability is not the biological reasonfor the endangered state of Rh. huadingense

     

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