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李 红, 郑春芳, 陈继浓, 于 冯, 康华靖. 不同温度下秋茄树幼苗叶绿素荧光参数对光强的响应[J]. 浙江林业科技, 2020, 40(2): 17-22. DOI: 10.3969/j.issn.1001-3776.2020.02.003
引用本文: 李 红, 郑春芳, 陈继浓, 于 冯, 康华靖. 不同温度下秋茄树幼苗叶绿素荧光参数对光强的响应[J]. 浙江林业科技, 2020, 40(2): 17-22. DOI: 10.3969/j.issn.1001-3776.2020.02.003
LI Hong, ZHENG Chun-fang, CHEN Ji-nong, YU Feng, KANG Hua-jing. Response of Chlorophyll Fluorescence Parameters of Kandelia obovata Seedlings to Light Intensity in Incubator with Different Temperature[J]. Journal of Zhejiang Forestry Science and Technology, 2020, 40(2): 17-22. DOI: 10.3969/j.issn.1001-3776.2020.02.003
Citation: LI Hong, ZHENG Chun-fang, CHEN Ji-nong, YU Feng, KANG Hua-jing. Response of Chlorophyll Fluorescence Parameters of Kandelia obovata Seedlings to Light Intensity in Incubator with Different Temperature[J]. Journal of Zhejiang Forestry Science and Technology, 2020, 40(2): 17-22. DOI: 10.3969/j.issn.1001-3776.2020.02.003

不同温度下秋茄树幼苗叶绿素荧光参数对光强的响应

Response of Chlorophyll Fluorescence Parameters of Kandelia obovata Seedlings to Light Intensity in Incubator with Different Temperature

  • 摘要: 为了更好地开展秋茄树Kandelia obovata 的引种、繁育等工作,以其2 年生移植容器苗为材料,设置了昼夜温度分别为A(25/20℃),B(20/15℃),C(15/10℃)和D(10/5℃)共4 个处理,研究了电子传递速率(ETR)、光系统II(PSII)反应中心的激发能捕获效率(Fv'/Fm')、PSII 反应中心电荷分离实际量子效率(ΦPSII)、光化学猝灭系数(qP)和非光化学猝灭系数(qN)等参数对光强(PAR)的响应曲线。结果表明,光响应机理模型和双指数模型可以较好地拟合不同温度处理下秋茄树幼苗ETR 对PAR 的响应,所得结果与实测值较为接近(P>0.05);在不同温度下,秋茄树幼苗叶片的饱和PAR 有所差异,在1 000 ~ 1 400 μmol·m-2·s-1 之间;以光响应机理模型拟合的结果为例,在A,B,C,D 不同处理下,秋茄树叶片的最大电子传递速率(ETRmax)间均有显著差异(P< 0.05),分别为124.393,110.812,64.153 和33.427 μmol·m-2·s-1;D 处理下的Fv'/Fm',ΦPSII,qP 和qN 均处于最低水平,说明5℃以下低温对秋茄树幼苗的光合影响较大。因此,建议在幼苗阶段,夏季进行适当遮阳,冬季喷施植物生长调节剂或采用其他措施进行保温。

     

    Abstract: In spring of 2017, container Kandelia ogovata seedlings were transplanted into plastic drum and in January 2019, experiments were conducted after 7 days slow rooting. Seedlings were treated in incubators with different day-night temperature such as 25/20℃ (A), 20/15℃ (B), 15/10℃ (C) and 10/5℃ (D). The results showed that the response of electron transfer rate (ETR) to the light intensity of different treated seedlings could be fitted well by the light response mechanism model and the double exponential model. The fitted values were close to the measured ones. The light saturation points of the leaves of different treated seedlings ranged from 1 000 μmol/m2/s to 1 400μmol/m2/s. Taking the result of model fitting by light response mechanism as an example, the maximum electron transfer rates of seedling leaves were significantly different at different temperatures (P<0.05), which were 124.393, 110.812, 64.153, and 33.427 μmol/m2/s, respectively. Seedligns with treatment of D had the lowest excitation energy capture efficiency of PSII (Fv'/Fm'), the actual photochemical quantum efficiency of PSII (ΦPSII), photochemical quenching coefficient (qP) and non-photochemical quenching coefficient (qN). It indicated that the low temperature had significant effect on the photosynthesis of K. ogovata seedlings.

     

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