Abstract: Experiment were conducted in September 2016 on difference of terpene lactones content in leaves of 20 introduced and selected clones of Ginkgo biloba, which were 7-years grafted on 15-18 year rootstocks in Baoji Ginkgo Germplasm Resources Garden, Shaanxi province. The results showed that leaf weight, leaf area, leaf length, leaf number on one tree, tree height and leaf water content were the main factors affecting terpene lactones content. Three clones were selected with better growth traits and total terpene lactones content.