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尹文秀, 张明哲, 楼成杰, 王金砖, 于文涛, 胡美玲, 吴 姗, 虞惠贞, 许 瑾, 张晓峰, 李明福. 基于ITS 序列的DNA 条形码技术鉴定红豆杉属植物[J]. 浙江林业科技, 2020, 40(2): 111-115. DOI: 10.3969/j.issn.1001-3776.2020.02.017
引用本文: 尹文秀, 张明哲, 楼成杰, 王金砖, 于文涛, 胡美玲, 吴 姗, 虞惠贞, 许 瑾, 张晓峰, 李明福. 基于ITS 序列的DNA 条形码技术鉴定红豆杉属植物[J]. 浙江林业科技, 2020, 40(2): 111-115. DOI: 10.3969/j.issn.1001-3776.2020.02.017
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YIN Wen-xiu, ZHANG Ming-zhe, LOU Cheng-jie, WANG Jin-zhuan, YU Wen-tao, HU Mei-ling, WU Shan, YU Hui-zhen, XU Jin, ZHANG Xiao-feng, LI Ming-fu. Identification of Species of Taxus by DNA Barcoding Based on ITS Sequence[J]. Journal of Zhejiang Forestry Science and Technology, 2020, 40(2): 111-115. DOI: 10.3969/j.issn.1001-3776.2020.02.017
Citation: YIN Wen-xiu, ZHANG Ming-zhe, LOU Cheng-jie, WANG Jin-zhuan, YU Wen-tao, HU Mei-ling, WU Shan, YU Hui-zhen, XU Jin, ZHANG Xiao-feng, LI Ming-fu. Identification of Species of Taxus by DNA Barcoding Based on ITS Sequence[J]. Journal of Zhejiang Forestry Science and Technology, 2020, 40(2): 111-115. DOI: 10.3969/j.issn.1001-3776.2020.02.017
shu

基于ITS 序列的DNA 条形码技术鉴定红豆杉属植物

Identification of Species of Taxus by DNA Barcoding Based on ITS Sequence

    摘要
  • 摘要: DNA 条形码技术是一种快速、简便、准确的物种鉴定方法。本研究利用DNA 条形码技术对南方红豆杉Taxuswallichiana var. maire 样品H1，H2，H3，H4；东北红豆杉T. cuspidata 样品D1，D2 和欧洲红豆杉T. baccata 样品EU1，EU2 八个样品的ITS1-18S 序列进行PCR 扩增和测序，将测序结果与GenBank 中的同源序列进行比对分析。结果表明，H1，H2，H3，H4 与南方红豆杉的序列相似性最高，D1，D2 与东北红豆杉T. cuspidata 的序列相似性最高，EU1，EU2 与欧洲红豆杉T. baccata 的序列相似性最高。用邻接法（NJ）构建ITS1-18S 序列的系统进化树（boostrap1 000 次重复），结果表明，H1，H2，H3，H4 和南方红豆杉聚为一枝；D1，D2 与东北红豆杉聚为一枝；EU1，EU2 与欧洲红豆杉聚为一枝。上述分析结果表明：H1，H2，H3，H4 为南方红豆杉；D1，D2 为东北红豆杉；EU1，EU2 为欧洲红豆杉，与采集地样品信息完全一致。故可以利用引物 ITS1-18S 从分子水平区分红豆杉属Taxus植物。

     

    Abstract: Fresh branches of Taxus were collected from 8 trees in Shanghai, Zhejiang and Jiangsu in March 2017. They were PCR amplified and sequenced by DNA barcoding, and the results were compared with the homologous sequences in GenBank. The results showed that 4 samples had the highest sequence similarity with T. wallichiana var. mairei. Two samples had the highest sequence similarity with T. cuspidata, and the other 2 samples with T. baccata. Phylogenetic tree established by neighbor-joining method based on ITS1-18S sequences indicated that 4 samples gathered with T. wallichiana var. mairei , 2 samples with T. cuspidat, and 2 samples with T. baccata. Analysis resulted that 4 samples were T. wallichiana var. mairei, 2 samples T. cuspidate and 2 samples T. baccata, which were completely consistent with the actual sampling tree species.

     

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