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潘 炘, 庄晓伟, 于海霞, 蒋应梯, 张文福, 王 进. 不同溶剂对枇杷落叶提取物的影响[J]. 浙江林业科技, 2020, 40(6): 18-23. DOI: 10.3969/j.issn.1001-3776.2020.06.003
引用本文: 潘 炘, 庄晓伟, 于海霞, 蒋应梯, 张文福, 王 进. 不同溶剂对枇杷落叶提取物的影响[J]. 浙江林业科技, 2020, 40(6): 18-23. DOI: 10.3969/j.issn.1001-3776.2020.06.003
PAN Xin, ZHUANG Xiao-wei, YU Hai-xia, JIANG Ying-ti, ZHANG Wen-fu, WANG Jin. Effect of Different Solvents on Extracts from Leaves of Eriobotrya japonica[J]. Journal of Zhejiang Forestry Science and Technology, 2020, 40(6): 18-23. DOI: 10.3969/j.issn.1001-3776.2020.06.003
Citation: PAN Xin, ZHUANG Xiao-wei, YU Hai-xia, JIANG Ying-ti, ZHANG Wen-fu, WANG Jin. Effect of Different Solvents on Extracts from Leaves of Eriobotrya japonica[J]. Journal of Zhejiang Forestry Science and Technology, 2020, 40(6): 18-23. DOI: 10.3969/j.issn.1001-3776.2020.06.003

不同溶剂对枇杷落叶提取物的影响

Effect of Different Solvents on Extracts from Leaves of Eriobotrya japonica

  • 摘要: 以自然衰老凋落的枇杷Eriobotrya japonica 叶片为原料,以水、乙醇、乙酸乙酯、正丁醇为溶剂,采用索式抽提,研究其提取物中多酚、总糖、花青素、黄酮类化合物的含量和得率,并分析其抗氧化能力。结果表明:4种溶剂中混合天然产物提取得率最高的为水提,其得率为22.1%;提取物中多酚得率和含量最高的溶剂分别为水和乙醇,分别为9.5%和0.509 mg·mg-1;提取物中总糖提取得率和含量最高的溶剂均为水,分别为4.97%和0.225mg·mg-1;乙醇对花青素的提取得率最高为1.08%,提取物中含量最高的为乙酸乙酯,为0.113 mg·mg-1;提取物中黄酮类化合物提取得率和含量最高的均为乙醇,分别为3.04%和0.210 mg·mg-1;抗氧化能力最强的为乙醇提取物,为0.446 μmol?mg-1。结论:自然衰老的枇杷叶具有较高的开发价值和广阔的应用前景,可依据总糖和总酚等不同组分提取目标选择适宜的溶剂体系。

     

    Abstract: In February and March 2019, fallen leaves were collected in Eriobotrya japonica orchard in Hangzhou, Zhejiang province. Experimentswere conducted on extraction by Sowhlet extractor method with different solvents such as water, ethanol, ethyl acetate and n-butanol. The resultsdemonstrated that the highest yield of mixed natural product was by water, topped to 22.1%. The highest extraction rate and content of polyphenolswas extracted by water and ethanol respectively, 9.5% and 0.509 mg/mg. The highest total carbohydrate rate and extract content was by water, 4.97 %and 0.225 mg/mg. The highest extraction rate of anthocyanin was 1.08 % in ethanol, and the highest extracted content was 0.113 mg/mg in ethylacetate. The highest extraction rate and content of total flavonoids was 3.04 % and 0.210 mg/mg in ethanol. Analysis on antioxidant capacity showedthat the best was ethanol extract, 0.446 μmol/mg.

     

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