Abstract: In August 2017, 8 fruiting bodies of wild Ganoderma lucidum were collected in Longquan, Zhejiang province and 8 strains were isolated. Experiments were conducted on cultivation of isolated stains on PDA and primary stock substrate for comparing their growth. The result showed that stain of LQ06 collected from Castanea mollissima had the fastest growth. Analysis on its morphology and sequences of the internal transcribed spacer (ITS) region demonstrated that LQ06 was G. lucidum Dai 2272. Cultivation tests of LQ06 were carried out in Longquan in 2018 and 2019, with different cut logs, under different canopy densities and green house. The result concluded that LQ06 cultivated on cut log of Quercus acutissima had the best growth and highest spores yield, with crown densities of 0.6 and 0.8. Cultivation in greenhouse could significantly increase the growth and spores yield. The average length of stipes and diameter of caps was 12.04 cm and 17.92 cm, with 5.63% of biological efficiency. The average weight of each dry fruiting body and spore yield was 163.43 g and 152.37 g, resulting in the ratio of 93.23%.