Abstract: From August 2018 to June 2019, investigations were conducted on flora and fauna resource in Deqing Xiaozhu Lake National Wetland Park, Zhejiang province. The results showed that there were 535 species of vascular plants, belonging to 126 families and 354 genera in the park, dominated by Asteraceae, Poaceae and Leguminosae. Among them, 3 species are under state grade 1 protection, 9 species under grade 2. There were 27 species of invasive plant. It has 6 vegetation type groups, 10 vegetation types and 73 formations. There were 25 species of large aquatic invertebrates of 2 phylum, 3 orders and 4 families, dominated by species of Unionidae. There had 242 species of vertebrates of 5 orders and 104 families, among them, 51 species of 17 families fish, dominated by Cyprinidae. Ranidae occupied the most part of 14 species of amphibians of 6 families. Reptiles had 19 species of 8 families, with the most species in Colubridae. There were 137 species of birds in 49 families, with the most species in passeriformes. Mammals had 21 species of 14 families, the most species was Mustelidae. 1 species of animal is under state 1 protection, Nipponia nippon, 7 species of under grade 2, and 8 species of invasive alien animals.