Abstract: From October 2016 to May 2017, investigations were made on resources of ancient and famous trees in Anji county, Zhejiang province, based on the initial survey in 1998-1999 and the supplementary survey in 2010-2012. The result showed that there were 3329 ancient and famous trees, accounting 77 species belonging to 33 families and 58 genera. Among them, 3290 were ancient trees and 41 famous trees. One Camellia sinensis ‘Baiye 1’and Parrotia subaequalis tree was both ancient and famous one. 48 trees were under state-grade one protection, 131 under state-grade two，3 111 under state-grade three. 33.33% and 27.08% ancient trees under state-grade one protection had weak and endangered growth. Countermeasures were conducted such as “one tree one method”.