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黄徐骏, 李海波, 陈友吾, 叶碧欢, 宋其岩, 胡传久, 沈建军, 廖荣俊. 基于多重荧光SSR标记鉴别榧树品种（品系）[J]. 浙江林业科技, 2021, 41(6): 24-29. DOI: 10.3969/j.issn.1001-3776.2021.06.004
引用本文: 黄徐骏, 李海波, 陈友吾, 叶碧欢, 宋其岩, 胡传久, 沈建军, 廖荣俊. 基于多重荧光SSR标记鉴别榧树品种（品系）[J]. 浙江林业科技, 2021, 41(6): 24-29. DOI: 10.3969/j.issn.1001-3776.2021.06.004
shu
HUANG Xu-jun, LI Hai-bo, CHEN You-wu, YE Bi-huan, SONG Qi-yan, HU Chuan-jiu, SHEN Jian-jun, LIAO Rong-jun. Identification of Cultivars of Torreya grandis Cultivars (Strains) by Multiple Fluorescent SSR Markers[J]. Journal of Zhejiang Forestry Science and Technology, 2021, 41(6): 24-29. DOI: 10.3969/j.issn.1001-3776.2021.06.004
Citation: HUANG Xu-jun, LI Hai-bo, CHEN You-wu, YE Bi-huan, SONG Qi-yan, HU Chuan-jiu, SHEN Jian-jun, LIAO Rong-jun. Identification of Cultivars of Torreya grandis Cultivars (Strains) by Multiple Fluorescent SSR Markers[J]. Journal of Zhejiang Forestry Science and Technology, 2021, 41(6): 24-29. DOI: 10.3969/j.issn.1001-3776.2021.06.004
shu

基于多重荧光SSR标记鉴别榧树品种（品系）

Identification of Cultivars of Torreya grandis Cultivars (Strains) by Multiple Fluorescent SSR Markers

    摘要
  • 摘要: 榧树Torreya grandis品种的准确鉴别对于新品种的选育与知识产权保护具有重要意义。为了建立高效鉴别榧树品种的分子技术手段，以9个榧树品种（品系）作为研究材料，通过对前期‘细榧’品种的转录组测序数据进行SSR标记筛选以获得可稳定扩增的品种（品系）间多态性SSR标记，从而揭示9个榧树品种（品系）间的基因型差异；进一步利用多重荧光SSR标记技术将多个多态性SSR标记整合，基于基因型组合的差异来高效鉴别榧树品种（品系）。结果表明，9个榧树品种（品系）的基因组DNA在SSR位点Tg_U7和Tg_U734可扩增出4种不同的基因型组合，分别为289/295和192/222、277/295和192/222、277/289/295和192/216/222以及277/295和192/216，可一次性将榧树中的‘玉山鱼榧’‘磐大榧’（品种）和大丁香（品系）鉴别出来。本研究结果表明基于多重荧光SSR标记技术的基因型组合差异可作为榧树品种（品系）特异性的SSR指纹，为榧树材料的鉴别提供了高效便捷的分子技术手段。

     

    Abstract: In November 2020, fresh tender leaves were collected from 6 cultivars and 3 strains (3 trees each) of Torreya grandis in Pan’an county, Zhejiang province for identification experiment. SSR markers were screened according to the previously established transcriptome database of a T. grandis ‘Xifei’ in order to obtain polymorphic SSR markers. These polymorphic SSR markers were further used for detect genotypic difference among 9 materials. The multiplex PCR assays were further established for the joint use of these polymorphic SSR markers for identifying cultivars and strains based on different genotype combinations. The results showed that 4 genotypes combinations including 289/295 plus 192/222, 277/295 plus 192/222, 277/289/295 plus 192/216/222, and 277/295 plus 192/216 were detected from two polymorphic SSR loci of Tg_U7 and Tg_U734 for 9 materials. The different genotype combinations allowed to identify simultaneously 2 cultivars and 1 strain of T. grandis, namely ‘Yushanyufei’, ‘Pandafei’ and Dadingxiang strain.

     

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