白鲜皮中3种柠檬苦素化合物抗松材线虫作用研究
Nematicidal Activity of Three Limonoids from Dictamnus dasycarps against Bursaphelenchus xylophilus
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摘要: 对白鲜Dictamnus dasycarpus 皮中3 种柠檬苦素类化合物（梣酮、黄柏酮、吴茱萸内酯）进行分离鉴定， 并采用浸渍法开展梣酮、黄柏酮、吴茱萸内酯毒杀松材线虫Bursaphelenchus xylophilus 研究。结果表明，梣酮、 黄柏酮、吴茱萸内酯对松材线虫均有一定的抑制作用，其对松材线虫的LC50 分别为9.78 μg·mL-1、15.99 μg·mL-1 和17.91 μg·mL-1，其中，梣酮在≥20 μg·mL-1 时，毒杀松材线虫的校正死亡率＞80%，杀线虫活性强。上述研究 结果显示，白鲜皮柠檬苦素类化合物具有开发为新型植物源杀松材线虫剂的潜力。Abstract: Three limonoids, namely Fraxinellone, Obacunone, Evodin were isolated from bark of Dictamnus dasycarps and were identified. Experiments were conducted on their nematicidal activity against Bursaphelenchus xylophilus. The results showed that Fraxinellone, Obacunone, Evodin had inhibitory effect on B. xylophilus. The LC50 for B. xylophilus was as follows: 9.78 μg/mL, 15.99 μg/mL and 17.91 μg/mL.
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