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张辉 , 徐端妙, 林秀君, 陈莉娟, 刘浩凯. 4种柏科植物挥发油对松材线虫的室内毒力测定[J]. 浙江林业科技, 2022, 42(2): 80-83. DOI: 10.3969/j.issn.1001-3776.2022.02.013
引用本文: 张辉 , 徐端妙, 林秀君, 陈莉娟, 刘浩凯. 4种柏科植物挥发油对松材线虫的室内毒力测定[J]. 浙江林业科技, 2022, 42(2): 80-83. DOI: 10.3969/j.issn.1001-3776.2022.02.013
ZHANG Hui, XU Duan-miao, LIN Xiu-jun, CHEN Li-juan, LIU Hao-kai. Nematicidal Activity of Essential Oil from Four Species of Cupressaceae against Bursaphelenchus xylophilus[J]. Journal of Zhejiang Forestry Science and Technology, 2022, 42(2): 80-83. DOI: 10.3969/j.issn.1001-3776.2022.02.013
Citation: ZHANG Hui, XU Duan-miao, LIN Xiu-jun, CHEN Li-juan, LIU Hao-kai. Nematicidal Activity of Essential Oil from Four Species of Cupressaceae against Bursaphelenchus xylophilus[J]. Journal of Zhejiang Forestry Science and Technology, 2022, 42(2): 80-83. DOI: 10.3969/j.issn.1001-3776.2022.02.013

4种柏科植物挥发油对松材线虫的室内毒力测定

Nematicidal Activity of Essential Oil from Four Species of Cupressaceae against Bursaphelenchus xylophilus

  • 摘要: 通过水蒸气蒸馏法提取日本花柏Chamaecyparis pisifera、日本扁柏C. obtusa、细叶花柏C. pisifera‘Plumoso’ 和台湾扁柏C. obtusa var. formosana 4 种柏科Cupressaceae 植物的挥发油,采用浸渍法研究4 种植物挥发油对松材 线虫Bursaphelenchus xylophilus 的毒杀作用。结果表明,4 种柏科植物挥发油对松材线虫均具有毒杀作用,抑制作 用呈现时间依赖性和浓度依赖性,日本花柏、日本扁柏、细叶花柏及台湾扁柏挥发油在浸渍72 h 时对松材线虫抑 制的LC50(半致死浓度)分别为2.84 mg·mL-1、2.77 mg·mL-1、2.37 mg·mL-1、1.76 mg·mL-1,其中台湾扁柏挥发 油对松材线虫的抑制作用最强。该研究为柏科植物挥发油资源的开发及其杀松材线虫天然活性物质的利用提供 依据。

     

    Abstract: Essential oils were extracted by steam distillation from four species of Cupressaceae, namely Chamaecyparis obtuse, Ch. pisifera ‘Plumoso’, Ch. pisifera, Ch. obtusa var. formosana for experiment of their nematicidal activity by immersion method against Bursaphelenchus xylophilu. B. xylophilus was treated with different concentrations of essential oils for 24 h, 48 h and 72 h. The results showed the inhibitory effect of essential oils from four species of Cupressaceae on B. xylophilus had positive relation with duration and concentration. The median lethal concentration (LC50) of four essential oils from Ch. obtuse, Ch. pisifera ‘Plumoso’, Ch. pisifera, Ch. obtusa var. formosana 72 h later was 2.84 mg/mL, 2.77 mg/mL, 2.37 mg/mL and 1.76 mg/mL respectively. The essential oil from Ch. obtusa var. formosana had the best effect.

     

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