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潘天乐, 刘凤霞, 毛毅斌, 刘建勋, 邹健强, 黄海军, 林盈, 杨仙玉, 张先福. 华南虎外周淋巴血细胞培养及染色体铺展分析[J]. 浙江林业科技, 2022, 42(6): 1-5. DOI: 10.3969/j.issn.1001-3776.2022.06.001
引用本文: 潘天乐, 刘凤霞, 毛毅斌, 刘建勋, 邹健强, 黄海军, 林盈, 杨仙玉, 张先福. 华南虎外周淋巴血细胞培养及染色体铺展分析[J]. 浙江林业科技, 2022, 42(6): 1-5. DOI: 10.3969/j.issn.1001-3776.2022.06.001
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PAN Tian-le, LIU Feng-xia, MAO Yi-bin, LIU Jian-xun, ZOU Jian-qiang, HUANG Hai-jun, LIN Ying, YANG Xian-yu, ZHANG Xian-fu. Culture of Peripheral Lymphocyte and Chromosome Analysis of Panthera tigris amoyensis[J]. Journal of Zhejiang Forestry Science and Technology, 2022, 42(6): 1-5. DOI: 10.3969/j.issn.1001-3776.2022.06.001
Citation: PAN Tian-le, LIU Feng-xia, MAO Yi-bin, LIU Jian-xun, ZOU Jian-qiang, HUANG Hai-jun, LIN Ying, YANG Xian-yu, ZHANG Xian-fu. Culture of Peripheral Lymphocyte and Chromosome Analysis of Panthera tigris amoyensis[J]. Journal of Zhejiang Forestry Science and Technology, 2022, 42(6): 1-5. DOI: 10.3969/j.issn.1001-3776.2022.06.001
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华南虎外周淋巴血细胞培养及染色体铺展分析

Culture of Peripheral Lymphocyte and Chromosome Analysis of Panthera tigris amoyensis

    摘要
  • 摘要: 华南虎Panthera. tigris amoyensis 是虎P. tigris 的八个亚种之一，为我国特有亚种。学界认为华南虎已在野 外灭绝，仅保有圈养繁育种群。为增加圈养华南虎繁殖量并为遗传性疾病诊断提供依据，本研究用人Homo sapiens 的外周血淋巴细胞培养基对华南虎外周血淋巴细胞进行培养，将培养好的细胞固定后进行染色体铺展，用胰酶消 化法进行G-显带。结果表明：华南虎染色体铺展良好，染色体数目为38 条，其染色体核型公式为：6（m）+6（sm） +3（st）+2（t），X,Y（m,m），其中第12 对为随体染色体；华南虎染色体G-显带带形特征分为6 型，其中A 型6 条、B 型8 条、C 型4 条、D 型8 条、E 型6 条、F 型4 条以及性染色体X、Y；但华南虎D3 染色体长臂缺 少一条深染带，C1 和F2 染色体发生了单体异态现象。上述结果表明，华南虎染色体结构在长期近亲交配中发生 了变化；华南虎D3 染色体长臂的缺少可能是导致卵母细胞质量下降或受精卵和胚胎发育异常，最终导致流产、 死胎以及出生缺陷等情况发生的重要原因。

     

    Abstract: Human peripheral blood lymphocyte culture medium was used to culture the peripheral Homo sapiens blood lymphocytes of Panthera tigris amoyensis, then the cultured cells fixed, the chromosomes spread, and G-banding by trypsin digestion. The results showed that there were 38 chromosomes, its karyotype formula was: 6 (m) +6 (sm) +3 (st) +2 (t), X, Y (m, m), of which the 12th pair was the satellite chromosome. G-banding characteristics P. tigris amoyensis chromosomes could be divided into 6 types, including 6 types A, 8 types B, 4 types C, 8 types D, 6 types E, 4 types F, and sex chromosomes X and Y. However, the long arm of D3 chromosome of P. tigris amoyensis lacks a hyper chromatic band, and monomeric heteromorphic occurs on C1 and F2 chromosomes.

     

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