Abstract: In November 2018, seeds were collected from 8 Bretschneidera sinensis trees in Xianxialing Provincial Nature Reserve of Zhejiang province for germination. In the spring 2019, geminated seeds were transplanted in containers, and in March 2020, container seedlings were transplanted in pot with different substrates, and yellow soil as contract. Determinations were carried out on net increment of seedling height, ground diameter, and also on growth traits of seedling with different treatment every 30 days. Chlorophyll a+b, soluble sugar and soluble protein content, MDA, SOD and POD content in leaves of seedling with different treatment was measured by the end of 30th, 60th and 90th days of treatment. The results showed that by the end of the experiment, height growth of treatment of yellow soil : sand = 9 : 1 and of yellow soil : sand = 8 : 2 was significantly higher than that of CK, and there was significant difference of leaf thickness among five treatments, while no significant difference of ground diameter and biomass. At different periods, there were differences among treatments in the contents of chlorophyll a+b, soluble sugar and soluble protein. MDA content and SOD enzyme activity of seedlings treated by yellow soil : sand = 8 : 2 were significantly lower than that by yellow soil : perlite=8 : 2 and CK by the end of 60th days and 90th days, indicating that treatment of yellow soil : sand = 8 : 2 was better for the growth of B.sinensis seedlings.