Abstract: From October 2018 to October 2019, investigations were conducted on mammals in Jiangshan Xianxialing Provincial Nature Reserve, Zhejiang province by grid method with infrared camera, rattrap and light trapping. The results showed that there were 63 species of mammals belonging to d 8 orders and 23 familie in the Reserve, including 5 species of 3 families of Eulipotyphla, 14 species of 3 families of Chiroptera, 2 species of 1 family of Primates, 1 species of 1 family of Pholidota, 1 species of 1 family of Lagomorpha, 17 species of 5 families of Rodentia, 18 species of 6 families of Carnivora, 5 species of 3 families of Artiodactyla. Compared with data in 2016, 8 species of mammals were recorded, namely Macaca thibetana , Rhinolophus luctus, Rh. Affinis, Rh. Pearsoni , Rh. Sinicus, Eptesicus serotinus , Myotis laniger and Scotomanes ornatus. Canis lupus, Vulpes vulpes, Cuon alpinus, Pardofelis temminckii, Neofelis nebulosi, Panthera pardus had not been recorded for nearly 30 years. Ursus thibetanus and Viverricula indica was captured by infrared camera for the first time in the Reserve. According to the living habits and habitats, the animals in the Reserve could be divided into epigaeic animal, dendrocole and flight animal. The geographical division was dominated by Oriental species, typical characteristics of Central China with mixed species and transition from Oriental to Palaearctic. There are 11 species of national key protected wild animals, including 3 species of national first class protected and 8 species of national second class protected.