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郑新建 , 宋唯真, 刘敏, 杨晓锋, 刘阳, 范忠勇, 吴迟庆. 浙江千岛湖海南鳽基因组测序组装与特征分析研究[J]. 浙江林业科技, 2023, 43(2): 22-27. DOI: 10.3969/j.issn.1001-3776.2023.02.004
引用本文: 郑新建 , 宋唯真, 刘敏, 杨晓锋, 刘阳, 范忠勇, 吴迟庆. 浙江千岛湖海南鳽基因组测序组装与特征分析研究[J]. 浙江林业科技, 2023, 43(2): 22-27. DOI: 10.3969/j.issn.1001-3776.2023.02.004
shu
ZHENG Xin-jian, SONG Wei-zhen, LIU Min, YANG Xiao-feng, LIU yang, FAN Zhong-yong, WU Chi-qing. Next-generation Sequencing and De Novo Assembly of Genome from Gorsachius magnificus in Thousand Island Lake[J]. Journal of Zhejiang Forestry Science and Technology, 2023, 43(2): 22-27. DOI: 10.3969/j.issn.1001-3776.2023.02.004
Citation: ZHENG Xin-jian, SONG Wei-zhen, LIU Min, YANG Xiao-feng, LIU yang, FAN Zhong-yong, WU Chi-qing. Next-generation Sequencing and De Novo Assembly of Genome from Gorsachius magnificus in Thousand Island Lake[J]. Journal of Zhejiang Forestry Science and Technology, 2023, 43(2): 22-27. DOI: 10.3969/j.issn.1001-3776.2023.02.004
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浙江千岛湖海南鳽基因组测序组装与特征分析研究

Next-generation Sequencing and De Novo Assembly of Genome from Gorsachius magnificus in Thousand Island Lake

    摘要
  • 摘要: 2017 年，通过采集浙江淳安1 只救助死亡的海南鳽Gorsachius magnificus 个体进行全基因组DNA 提取， 使用二代测序及重头组装技术，首次开展海南鳽基因组测序组装，并将其与鹈形目Pelecaniformes 中的白鹭Egretta garzetta、朱鹮Nipponia nippon、卷羽鹈鹕Pelecanus crispus、普通鸬鹚Phalacrocorax carbo 这四种鸟类进行比较 基因组学分析研究。结果表明：（1）组装测得海南鳽的全基因组，获得基因组大小为1.14 Gbp，重叠群N50 指 标为77.8 kbp，大片段N50 指标为1.95 Mbp，这些指标说明海南鳽基因组大小符合预期，组装质量较为理想；（2） 对该个体全基因组从头测序，获得72 Gbp 的碱基数据得到约56X 测序深度；（3）比较基因组分析表明，海南鳽 与白鹭、朱鹮的系统关系更为接近，与卷羽鹈鹕、普通鸬鹚的进化关系较远；（4）在基因的适应性方面，海南鳽 享有大量特有的基因，同时与其他夜行性鸟类一样，在感官、神经及昼夜节律调节上与其他昼行性鸟类有较大的 分化。本研究从遗传上初步证实海南鳽是一种演化上非常特化的夜行性鸟类。

     

    Abstract: In 2017, one rescued dead Gorsachius magnificus in Thousand Island Lake, Zhejiang province was collected for genome DNA extraction by next-generation sequencing and de novo assembly technique. The results showed that genome size was of G. magnificus 1.14Gbp. The N50 index of overlapping group was 77.8 Kbp, and the large fragment was 1.95 Mbp, indicating that the genome size could be de novo assemble. The whole-genome of 72Gbp was obtained, and the sequencing depth was about 56X. The phylogenetic relationship of G. magnificus was closer with Egretta garzetta Nipponia nippon than Pelecanus crispus and Phalacrocorax carbo. Comparative genomic analysis demonstrated that in terms of nocturnal adaptability, G. magnificus had a large number of unique genes. Like other nocturnal birds, it had a large number of candidate genes from diurnal birds in sensory, neurological and circadian rhythm regulation.

     

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