Abstract: On April 23, 2021, volatiles contents were collected from Pittospora tobira branch during blooming stage at 09:00, 13:00 and 18:00 in Hangzhou, Zhejiang province for determination. On April 24, 2021, experiments on smelling volatiles from a potted P. tobira during blooming stage were conducted on human beings with brain waves (θ wave, α wave, SMR wave, β wave and γ wave), blood pressure (systolic and diastolic blood pressure) and heart rate as physiological index, and profile of mood state scale (POMS) and state trait anxiety inventory (S-AI) as psychological index. The results showed that smelling the volatiles of P. tobira for 30 minutes had good effect of physiological and psychological recovery. It could calm the physical state, improve the mental vitality of the brain, as well as could reduce the negative state. Meanwhile, the experiment demonstrated that the effect of smelling had difference between male and female, the latter had lager effect. The research also found that the daily change of plant volatiles would affect the effect of human health, showed that it had the strongest effect at night.