Abstract: In March 2019, 1-year Populus×canadensis‘Zhongqian 3’ seedlings was introduced from Qianjiang of Hubei province in Longquan, Zhejiang province for scion. Seedling cultivation by cuttings of 20-25 cm was carried out in different sample sites with different elevation in 2019, and topped seedlings were afforestated with two specifications in 2020. In November 2021, determinations were implemented on height, DBH and crown diameter, meanwhile, sample trees were selected for biomass detection. The result showed that elevation and soil fertility had great influence on the height growth of seedlings, while the ground diameter growth was greatly affected by soil fertility and less affected by elevation. There was a significant difference in seedling height growth among different months (P< 0.01), and there was no significant difference in ground diameter growth between adjacent months, but there was a significant difference between the beginning and ending months of the growth period (P< 0.01). The growth of seedling at lower elevation had the best effect with the height and ground diameter of superior seedlings of 4.6 m and 3.0 cm. The average height and ground diameter of seedlings were 3.9 m and 3.0 cm. The experiment demonstrated that the tree height, DBH, crown width and biomass of larger seedlings with ground diameter of more than 2.5 cm and height of more than 300 cm after afforestation were better than that of small ones.