Abstract: In July 2021, bitter shoots of Bambusa pervariabilis, B. cornigera , Dendrocalamus farinosus, B. rigida, B. distegia, Neosinocalamus affinis and B. changningensis were harvested from Century Bamboo Garden in Changning, Sichuan province. Determinations were carried out on content of protein and 16 free amino acids. Evaluation was made on amino acid score (AAS) and amino acid chemical score (CS) reflecting the protein nutritional value, and analysis was made on the ratio coefficient (RC) and ratio coefficient score (SRC) of amino acid. The results showed that the protein content in tested bitter bamboo shoots varied from 1.56 to 2.46 g·100 g-1, and the highest was in N. affinis, 2.46 g·100 g-1. The total amino acid content of the tested bitter bamboo shoot ranged from 1 164.33 to 1722.67 mg/100 g. Leu content was the highest essential amino acid in the seven bamboo shoots, and Asp content was the highest non-essential amino acid. With reference to AAS and CS, the nutritional value of N. affinis was better than that of the left shoots. However, based on the analysis of RC, SRC and EAA/TAA values, the essential amino acid composition of B. rigida was more reasonable than other species.