Abstract: On April 30, 2022, 3-year seedlings of Cinnamomum japonicum var. chenii, Machilus thunbergia and Neolitsea sericea were potted in Zhoushan, Zhejiang province. Experiment was conducted on fertilization of different quantity of organic fertilizer (Y), compound fertilizer (F) and mixture of organic fertilizer and compound fertilizer (YF), with non fertilizer as CK on May 8, 2022. On September 22, 2022, the test finished and determination was implemented on height and ground diameter of seedlings, as well as on soil physio-chemical properties and nutrient content in different organs of seedlings. The results revealed that seedling treated by organic fertilizer (6 g/plant) had the best effect of height and ground diameter growth of C. japonicum var. chenii’s (increased by 7.54 and 95.07 % compared with CK). For M. thunbergia and N. sericea, the mixture fertilizer (3 g/plant) had the best effect on height and ground diameter. Accumulation of N and P of tested seedlings occurred mainly in root and leaves. In contrast, the accumulation of K only occurred in root. Accumulation of N at different organs of seedlings treated by compound fertilizer and mixture fertilizer was larger than that treated by organic fertilizer and CK. P content in treated seedling had no great difference with that in the control. K content at root of C. japonicum var. chenii treated by compound fertilizer of 9 g/plan was evidently higher than that of CK. Correlation analysis and mantel test resulted that the soil pH had negative relation with growth of M. thunbergia (P＜0.01). The N content in soil had positive relation with height and ground diameter growth of three species (P＜0.01). The effect of P and K content had no great effect on growth. The result indicated that organic fertilizer of 6 g/plant had the best effect for C. japonicum var. chenii, whereas mixture of 3 g/plant fertilizer for M. thunbergia and N. sericea.