Abstract: In June 2020, broadcast preparation of oil was carried out under pure Cunninghamia lanceolata stand planted in 1988 in Jingning, Zhejiang province. In July 2020, wild 1-2 year Polygonatum cyrtonema rhizomes were cut for experiment of 6-Benzylaminopurine (6-BA), indoleacetic acid (IAA), gibberellin (GA3) and mixed solution of the three treatment and seedling cultivation by single factor. In April 2021, investigations were implemented on germinations, new root, seedlings, height and ground diameter and seedling leaves of cut. The result demonstrated that different plant hormone treatments had significant effect on rhizome germination, new root, emergence rate, seedling height, ground diameter and leaf number (P <0.01). The cut treated 1 hour with mixture of 150 mg/L of 6BA + 100 mg/L of IAA mixture, then 20 minutes with 42 500-fold GA3, 3 hours with mixture of 150 mg/L of 6BA + 100 mg/L of IAA, then 20 minutes with 42 500-fold GA3, 2 hours of solution of 150 mg/L of 6BA, had significantly more rhizome germination, seedling emergence rate and seedling growth. Each cut had average 1.29-1.82 new shoot in rhizomes, 3.48- 6.92 new root, 67.59%-86.74% emergence rate, 0.21-0.22 cm ground diameter, 19.56-21.43 cm height, 5.43- 6.19 leaves. Compared with CK (treated by water), the number of new buds increased by 26.47%-78.43%, number of new roots by 40.32-179.03%, emergence rate by 21.25%-87.18%, ground diameter by 23.53%-29.41%, height by 13.79%-24.67%, number of leaves by 5.23%-19.96%.