Abstract: Based on literatures and field investigations during 2018 and 2019, seed plant in different counties of Lishui, Zhejiang province was listed. The results show that there were 2 785 species of wild seed plants belonging to 921 genera and 160 families in Lishui. The largest number of species was in Qingyuan county, with 2 069 species belonging to 784 genera and 155 families, accounting for 96.88%, 85.12% and 74.29% of that in Lishui, and followed by Longquan. At the family floristic element, the ratio of tropical and temperate zone in Lishui was 2.00, and the ratio of counties ranged between 1.84 and 2.09, indicating that the section level was mainly of tropical in nature. At the genera floristic element, the tropical/temperate value of Lishui was 0.83, and that of each county was 0.75-0.89, indicating that the temperate of the genera was slightly dominant. In terms of floristic division, it is generally bounded by Yunhe and Songyang, which could be divided into Western medium mountain area (I area) and Eastern low mountain area (II area). Area I could be divided into Qingyuan, Longquan sub area, Suichang sub area and Jingning sub area, and area II into Yunhe-Qingtian-Jinyun sub area, Songyang sub area and Liandu sub area. Each sub area was interrelated and had own properties, reflecting the complexity and diversity of Lishui seed plant flora. Longquan-Qingyuan sub area and Jingning sub area had the highest species diversity index.