Abstract: Through field investigation and consulting literature, the fern flora composition and geographical composition with Shexiang Caoyutang National Forest Park in Zhejiang Province were analyzed, and meanwhile differentiation strength and similarity coefficient among the flora and other 4 floras were compared. The results showed that: (1) there are 97 species of pteridophytes belonging to 45 genera and 29 families in Shexiang Caoyutang National Forest Park. The dominant composition of families and genera is obvious. Predominant families are Dryopteridaceae, Thelypteridaceae, Polypodiaceae, Selaginellaceae, Athyriaceae and Aspleniaceae, and predominant genera are Dryopteris, Selaginella, Asplenium, Arachniodes, Cyclosorus and Lepisorus. (2) The geographical composition of the flora are mainly tropical attributes at the level of families and genera, and mainly temperate attributes at the level of species. The fern flora has the characteristics of infiltration and the transition from tropical to temperate area. (3) Comparative analysis with 4 nearby ferns show that Caoyutang Forest Park has lower differentiation strength and less flora richness. And in terms of the phylogenetic relationship, Caoyutang Forest Park has the closest relationship with Zijin Mountain, followed by Ganjiangyuan, and the relationship with Mayang River and Xingdou Mountain is relatively distant.