Abstract: In October 2019, Polygonatum cyrtonema seeds were collected in Lin’an, Zhejiang province. After one year of storage, seeds were used for experiment of germination with treatment of 0, 0.5, 1.0 and 2.0 mg/L NAA in 1/2MS. 50 days later, germination rate was calculated. While cultivation of rhizome on the same medium. 80 days later, root length, diameter and number was determined. The results showed that seed germination rate of each treatment ranged from 20%-30%. With the increase of NAA concentration, the number of roots derived from P. cyrtonema seed increased 11, 13 and 16 more, and root length shortened 10, 15 and 19 cm compared with that treated by 0 （control）. While the number of roots derived from P. cyrtonema rhizome increased 15, 31 and 36 more, and root length shortened 4, 6 and 7 cm compared with that of the control. Root diameter increased by 300, 500 and 800μm.