Abstract: In order to obtain a comprehensive understanding of the vascular plant diversity and floristic composition in the Daluo Mountain area in Wenzhou, Zhejiang Province, extensive field investigations were conducted, supplemented by relevant literature and specimen reviews, resulting in a checklist of vascular plants from Daluo Mountain area. The findings revealed that there was a total of 529 species belonging to 114 families and 341 genera, including 16 species of gymnosperms from 6 families and 11 genera, 484 species of angiosperms from 95 families and 312 genera, and 29 species of ferns from 13 families and 18 genera. Monogeneric families and monospecific genera accounted for 47.37% of the total families and 71.26% of the total genera, respectively. At both family and genus levels of seed plants, pantropical distribution types were predominant, with tropical distributions significantly exceeding temperate ones. At the family and genus levels of the ferns, the pantropical distribution was also dominated. The above indicated that the floristic composition of vascular plants in the Daluo Mountain area exhibited distinct subtropical characteristics. Additionally, the survey identified 39 invasive plant species, with Asteraceae being the dominant family, and two species of ClassⅡstate key protected wild plants, Fagopyrum dibotrys and Actinidia chinensis.