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4种微塑料对观赏草生长及生理特性的影响

Effects of Four Types of Microplastics on the Growth and Physiological Characteristics of Ornamental Grasses

  • 摘要: 为筛选出适用于不同微塑料 (Microplastics,MPs)污染地区治理的园林植物,本文评估聚乙烯(PE)、聚苯乙烯(PS)、聚乳酸(PLA)和聚对苯二甲酸-己二酸丁二醇酯(PBAT)4种不同类型MPs污染土壤对矮蒲苇Cortaderia selloana、狼尾草Pennisetum alopecuroides和细叶芒 Miscanthus sinensis 3种观赏草生长及生理特性的影响。通过测定3种观赏草在4种不同类型MPs污染土壤上栽种90 d后的株高和生物量,直观地反映植物生长受到的影响程度,同时测定叶绿素含量、可溶性糖含量和细胞膜透性,了解其抗逆能力。结果表明:①污植株株高基本下降,3种观赏草的生物量除PS污染土壤种植的观赏草外,其余MPs种植的观赏草均呈下降趋势;②植株的叶绿素、叶绿素a、叶绿素b含量均表现为矮蒲苇经PLA污染土壤种植后上升,狼尾草经PBAT污染土壤种植后下降,其余处理无显著变化;③矮蒲苇经PE和PBAT污染土壤种植后及狼尾草经PBAT污染土壤种植后均表现为可溶性糖含量升高,狼尾草经PS污染土壤种植后及细叶芒经4种MPs污染土壤种植后可溶性糖含量均表现为下降趋势;④3种植物经4种MPs污染土壤种植后细胞膜透性基本无显著变化,仅PLA处理细叶芒后显著升高。总体而言,不同类型MPs对同一植物同一指标影响可能不同,且同一种MPs对不同种类植物的同一指标的影响亦可能不同;可生物降解MPs对植物生长的影响可能高于传统的MPs。

     

    Abstract: To screen out garden plants suitable for soils contaminated with different types of microplastics (MPs), this study evaluated the effects of soil contamination by four types of MPs, i.e., polyethylene (PE), polystyrene (PS), polylactic acid (PLA), and polybutylene adipate-co-terephthalate (PBAT) on the growth and physiological characteristics of three ornamental grasses, namely, Cortaderia selloana, Pennisetum alopecuroides, and Miscanthus sinensis. Plant height and biomass of the three grasses were measured after growth of 90 days in soils contaminated with the MPs, directly reflecting the degree of growth impact; meanwhile, chlorophyll content, soluble sugar content and cell membrane permeability were determined to evaluate their stress resistance. The results showed that: (1) The plant height generally decreased, the biomass of three grasses showed a decreasing trend under all MPs treatments except that of the grass grown in the PS contamination. (2) Chlorophyll, chlorophyll a, and chlorophyll b contents of C. selloana exhibited an increase when grass grew in the PLA contamination, whereas those of P. alopecuroides showed a decrease under the PBAT contamination, and no significant changes were observed in the remaining treatments. (3) For C. selloana grown in the PE-and PBAT-contaminated soil and P. alopecuroides grown in the PBAT-contaminated soil, their soluble sugar contents increased; whereas for P. alopecuroides grown in the PS-contaminated soil and M. sinensis grown in the soil contaminated with all four types of MPs, their soluble sugar contents showed a decreasing trend. (4)The cell membrane permeability of the three grasses grown in the soils contaminated with four types of MPs showed generally no significant change, except for a notable increase in M. sinensis after the PLA contamination. In conclusion, different types of MPs may exert different effects on the same indicator of the same plant, and the same MP may also differentially affect the same indicator of different plant species. Additionally, the impact of biodegradable MPs on plant growth may be greater than that of conventional MPs.

     

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