Abstract:
In order to shorten the breeding process of
Viburnum plants, mature embryos of
Viburnum taitoense ×
Viburnum suspensum were used as explants for tissue culture and rapid propagation research. The results showed that the combined treatment of 15 seconds’ 70% ethanol disinfection followed by 0.1% mercuric chloride for 25 min was the most effective for sterilization of
V.
taitoense ×
V.
suspensum seeds, which not only ensured a high germination rate, but also controlled the pollution rate below 7%. The optimal medium for the start-up culture was 1/2 MS + 0.3 g·L
-1 lactalbumin hydrolysate, achieving a germination rate of 91.95%. The best multiplication culture medium was 1/2MS+0.5 mg·L
-16-BA+0.2 g·L
-1 IBA+0.3 g·L
-1lactalbumin hydrolysate, yielding a multiplication coefficient of 4.83. The optimal rooting culture medium was 1/2 MS + 0.5 mg·L
-1 IBA+ 0.05 mg·L
-1 NAA, resulting in a rooting rate of 90.28%. In this study, the in vitro culture and rapid propagation technology system of distant hybrid embryos of
Viburnum is established. This system greatly promotes the efficiency of hybrid breeding o
f Viburnum species and accelerates the breeding process, and also provides a valuable reference for research on embryo culture of other woody ornamental plants.