高级检索+

台东荚蒾×琉球荚蒾胚离体培养及快繁技术

Embryo Culture in Vitro and Rapid Propagation Technology of Viburnum taitoense ×Viburnum suspensum

  • 摘要: 为缩短荚蒾属(Viburnum)植物育种进程,本研究以台东荚蒾(Viburnum taitoense)×琉球荚蒾(Viburnum suspensum)成熟胚为外植体,开展组培快繁研究。结果表明,70%乙醇消毒15 s和0.1%氯化汞消毒25 min的混合处理最适合台东荚蒾×琉球荚蒾种子的灭菌,既能保证萌芽率,又能将污染率控制在7%以内;1/2MS+水解乳蛋白0.3 g·L−1是台东荚蒾×琉球荚蒾种子启动培养的适宜培养基,萌芽率为91.95%;增殖培养以1/2MS+0.5 mg·L−1 6-BA+0.2 mg·L−1 IBA+0.3 g·L−1水解乳蛋白最佳,增殖系数达4.83;生根培养以1/2MS+0.5 mg·L−1 IBA+0.05 mg·L−1 NAA最佳,生根率达90.28%。本研究建立了荚蒾属植物远缘杂交胚离体培养及快繁技术体系,对提高荚蒾属植物杂交育种效率、加快育种进程具有极大的促进作用,对其他木本观赏植物胚离体培养技术的研究也具有一定的参考价值。

     

    Abstract: In order to shorten the breeding process of Viburnum plants, mature embryos of Viburnum taitoense × Viburnum suspensum were used as explants for tissue culture and rapid propagation research. The results showed that the combined treatment of 15 seconds’ 70% ethanol disinfection followed by 0.1% mercuric chloride for 25 min was the most effective for sterilization of V. taitoense × V. suspensum seeds, which not only ensured a high germination rate, but also controlled the pollution rate below 7%. The optimal medium for the start-up culture was 1/2 MS + 0.3 g·L-1 lactalbumin hydrolysate, achieving a germination rate of 91.95%. The best multiplication culture medium was 1/2MS+0.5 mg·L-16-BA+0.2 g·L-1 IBA+0.3 g·L-1lactalbumin hydrolysate, yielding a multiplication coefficient of 4.83. The optimal rooting culture medium was 1/2 MS + 0.5 mg·L-1 IBA+ 0.05 mg·L-1 NAA, resulting in a rooting rate of 90.28%. In this study, the in vitro culture and rapid propagation technology system of distant hybrid embryos of Viburnum is established. This system greatly promotes the efficiency of hybrid breeding of Viburnum species and accelerates the breeding process, and also provides a valuable reference for research on embryo culture of other woody ornamental plants.

     

/

返回文章
返回