Abstract: Long-term experiment was conducted on application of B, Zn and Cu fertilizer at Carya cathayensis forest in Lin’an, Zhejiang province from 2012 to 2014. Determinations were implemented on B, Zn and Cu content in leaf, as well as pollen viability and fruit yield. Results showed that fertilizer treatments could increase B and Zn content in leaf by 5.3%-46.2% and 2.3-44.5%, compared to the control（no treatment）, but had evident effect on Cu content. B, Zn and Cu content in leaf of treated stand decreased from April to September, ordered by Cu > Zn > B. B and Zn fertilizer could improve pollen viability and increase fruit yield, especially in the second year of application. The experiment demonstrated that B and Zn fertilizer had much better effect than Cu on leaf nutrition, pollen viability and fruit yield.