人工绘制品种鉴别图法鉴定榧树属植物
Identification of Torreya Arn. by MCID Method
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摘要: 以19个榧树属植物的嫩叶为试验材料,采用改良CTAB法进行DNA提取,选用11个碱基组成的随机引物并筛选退火温度对其进行随机扩增的DNA多态性(RAPD)分析,根据特异性谱带构建相应的图谱关系,通过人工绘制品种鉴别图法(MCID),快速、准确鉴别这19个榧树属植物。Abstract: Random primers with the composition of 11 bases were selected for RAPD amplification of 19 species and cultivars of Torreya Arn., and corresponding map relationships were built according to the specific bands. Species were identified quickly and efficiently through drawing the diagram manual cultivar identification diagram (MCID).