Abstract: Experiments were conducted in 2013 on germination of Rosa multiflora ‘Lushuohong’ seeds collected in October, November and December of 2012 and treated by different methods. The result demonstrated that it had different effect of treatments on germination rate, germinating energy and germination index. The results showed that different treatment methods had different effect on germination rate, germination potential and germination index of seed collected in the same month. Seeds treated by pulse water, mechanical breaking seed coat and cold stratification could improve seed germination rate, especially the first treatment could increase germination rate and potential and germination index. Tests on treatment of pulse water on seeds of different collected time showed that germination rate, potential and index was the highest for seeds collected in October.