Abstract: Experiments were conducted on treatment of different soil water content on height and ground diameter growth, moisture content in leaf, biomass, chlorophyll, MDA and soluble sugar in 1-year Cyclocarya paliurs seedlings. The result demonstrated that with decrease of soil water content, height and ground diameter growth and above ground biomass decreased, underground biomass and total one increased first, decreased later, and root-shoot ratio increased. The total biomass of seedlings topped in soil with 50%-60% water content of field moisture capacity. With decrease of soil water content, moisture content in seedling leaf decreased, chlorophyll content increased first, decreased later, MDA and soluble sugar content increased. Chlorophyll content in seedlings leaf reached the highest in soil with 50%-60% water content of field moisture capacity. The experiments resulted that it had no evident difference of tested items among different treatments, except the one in soil with 15%-25% soil moisture of field moisture capacity, indicating seedling could grow well except the last one.